Active Recombinant Human PLA2G2A Protein (1-124; variant N1A)

• Cat.No.: PLA2G2A-12H
• Product Overview: Active recombinant human sPLA2 (Type IIA) protein (1-124; variant N1A) without tag was expressed in E. coli

Specification

• Description: The protein encoded by this gene is a member of the phospholipase A2 family (PLA2). PLA2s constitute a diverse family of enzymes with respect to sequence, function, localization, and divalent cation requirements. This gene product belongs to group II, which contains secreted form of PLA2, an extracellular enzyme that has a low molecular mass and requires calcium ions for catalysis. It catalyzes the hydrolysis of the sn-2 fatty acid acyl ester bond of phosphoglycerides, releasing free fatty acids and lysophospholipids, and thought to participate in the regulation of the phospholipid metabolism in biomembranes. Several alternatively spliced transcript variants with different 5' UTRs have been found for this gene.
• Source: E. coli
• Species: Human
• Tag: Non
• Molecular Mass: 13.9 kDa
• Protein length: 1-124; variant N1A
• Purity: ≥ 85 % estimated by SDS-PAGE
• Unit Definition: One unit is defined as the amount of enzyme required to hydrolyze 1 nmol of diheptanoyl thio-PC per minute at 37 centigrade in 25 mM Tris-HCl, pH 7.5, containing 10 mMcalcium chloride, 100 mM potassium chloride, 0.3 mM Triton X-100, 0.44 mM DTNB, and 1.48 mM diheptanoyl thio-PC.
• Stability: ≥ 1 year
• Storage: At -80 centigrade
• Storage Buffer: 50 mM Tris-HCl, pH 7.6, with 100 mM sodium chloride and 10 mM calcium chloride.
• Shipping: Dry ice in continental US; may vary elsewhere

Gene Information

• Gene Name: PLA2G2A phospholipase A2 group IIA [ Homo sapiens (human) ]
• Official Symbol: PLA2G2A
• Synonyms: PLA2G2A; phospholipase A2 group IIA; MOM1; PLA2; PLA2B; PLA2L; PLA2S; PLAS1; sPLA2; phospholipase A2, membrane associated; GIIC sPLA2; NPS-PLA2; group IIA phospholipase A2; non-pancreatic secretory phospholipase A2; phosphatidylcholine 2-acylhydrolase 2A; phospholipase A2, group IIA (platelets, synovial fluid); EC 3.1.1.4
• Gene ID: 5320
• mRNA Refseq: NM_000300
• Protein Refseq: NP_000291
• MIM: 172411
• UniProt ID: P14555

Reviews

11/23/2022

PLA2G2A's ability to easily attach to the surface of a cell or material.

06/14/2022

It has the characteristics of high safety in clinical treatment.

04/30/2022

The operation is very simple, get started quickly, easy to use, will recommend to others.

Q&As

How to use the expression pattern of PLA2G2A protein for disease diagnosis?

By detecting the expression pattern of PLA2G2A proteins, it can assist in the diagnosis of certain diseases, such as inflammatory diseases, cardiovascular diseases, cancer, etc.

What is the role of PLA2G2A proteins in the immune system?

PLA2G2A proteins have the role of regulating inflammatory response and apoptosis in the immune system, which can affect the survival and function of immune cells.

How to study the regulatory mechanisms of PLA2G2A proteins?

Studying the regulatory mechanism of PLA2G2A proteins requires a comprehensive use of various experimental methods and techniques, such as gene knockout, transcriptome analysis, and protein-protein interactions.

Is the expression of PLA2G2A protein related to the stage of disease development?

The expression of PLA2G2A protein may be related to the stage of the disease, and the expression level of the PLA2G2A protein may change at different stages of the disease development.

What are the types of mutations in PLA2G2A proteins?

The types of mutations in PLA2G2A proteins include point mutations, insertions/deletions, duplications, etc., which can lead to structural and functional abnormalities of the protein.

What is the relationship of PLA2G2A protein to other genes or proteins?

PLA2G2A proteins have complex relationships with other genes or proteins, and can interact with other genes or proteins and participate in a variety of biochemical reactions.