Recombinant Human, PLA2G2A His-tagged

• Cat.No.: PLA2G2A-1036H
• Product Overview: Total 183AA. MW: 20.6kDa (calculated). N-Terminal His-tag, 16 extra AA (highlighted).

Specification

• Cat. No.: PLA2G2A-1036H
• Description: Phospholipase A2, membrane associated is an enzyme that in humans is encoded by the PLA2G2A gene.
• Source: E. coli
• Species: Human
• Form: Filtered (0.4 um) and lyophilized in 0.5 mg/ml in 0.05M Acetate buffer pH 4.
• Researchtopic: Secreted phospholipases A2
• Purity: >95%
• Reconstitution: Add 0.1M Acetate buffer pH4 to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10 ug/ml. In higher concentrations the solubility of this antigen is limited. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
• Applications: Western blotting
• QualityControl Test: BCA to determine quantity of the protein; SDS PAGE to determine purity of the protein.
• Sequences of aminoacids: MRGSHHHHHH GMASHMQEQA QTTDWRATLK TIRNGVHKID TYLNAALDLL GGEDGLCQYK CSDGSKPFPR YGYKPSPPNG CGSPLFGVHL NIGIPSLTKC CNQHDRCYET CGKSKNDCDE EFQYCLSKIC RDVQKTLGLT QHVQACETTV ELLFDSVIHL GCKPYLDSQR AACRCHYEEK TDL
• Storage: Store lyophilized protein at -20°C. Lyophilized protein remains stable until the expiry date when stored at -20°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after one week at 4°C.

Gene Information

• Gene Name: PLA2G2A phospholipase A2, group IIA (platelets, synovial fluid) [ Homo sapiens ]
• Official Symbol: PLA2G2A
• Synonyms: PLA2G2A; phospholipase A2, group IIA (platelets, synovial fluid); MOM1; PLA2; PLA2B; PLA2L; PLA2S; PLAS1; sPLA2; OTTHUMP00000002786; Phospholipase A2, membrane associated; EC=3.1.1.4; Group IIA phospholipase A2; GIIC sPLA2; Non-pancreatic secretory phospholipase A2; NPS-PLA2; Phosphatidylcholine 2-acylhydrolase 2A; RASF-A; EC 3.1.1.4
• Gene ID: 5320
• mRNA Refseq: NM_000300
• Protein Refseq: NP_000291
• MIM: 172411
• Uniprot ID: P14555
• Chromosome Location: 1p35
• Function: calcium ion binding; calcium-dependent phospholipase A2 activity; hydrolase activity; phospholipase A2 activity; phospholipid binding

Reviews

11/23/2022

PLA2G2A's ability to easily attach to the surface of a cell or material.

06/14/2022

It has the characteristics of high safety in clinical treatment.

04/30/2022

The operation is very simple, get started quickly, easy to use, will recommend to others.

Q&As

How to use the expression pattern of PLA2G2A protein for disease diagnosis?

By detecting the expression pattern of PLA2G2A proteins, it can assist in the diagnosis of certain diseases, such as inflammatory diseases, cardiovascular diseases, cancer, etc.

What is the role of PLA2G2A proteins in the immune system?

PLA2G2A proteins have the role of regulating inflammatory response and apoptosis in the immune system, which can affect the survival and function of immune cells.

How to study the regulatory mechanisms of PLA2G2A proteins?

Studying the regulatory mechanism of PLA2G2A proteins requires a comprehensive use of various experimental methods and techniques, such as gene knockout, transcriptome analysis, and protein-protein interactions.

Is the expression of PLA2G2A protein related to the stage of disease development?

The expression of PLA2G2A protein may be related to the stage of the disease, and the expression level of the PLA2G2A protein may change at different stages of the disease development.

What are the types of mutations in PLA2G2A proteins?

The types of mutations in PLA2G2A proteins include point mutations, insertions/deletions, duplications, etc., which can lead to structural and functional abnormalities of the protein.

What is the relationship of PLA2G2A protein to other genes or proteins?

PLA2G2A proteins have complex relationships with other genes or proteins, and can interact with other genes or proteins and participate in a variety of biochemical reactions.