Active Recombinant Human PIK3CB/PIK3R1 protein, His-tagged

• Cat.No.: PIK3CB&PIK3R1-155H
• Product Overview: Recombinant full-length human p110 beta and human p85 alpha were co-expressed by baculovirus in Sf9 insect cells using an N-terminal His tag on both proteins.

Specification

• Description: The PI3K comprises of a 110 kDa catalytic subunit and an 85 kDa regulatory subunit. A number of isoforms of the 110 kDa catalytic subunit and the 85 kDa regulatory subunit exist in cells. The p110 beta catalytic subunit (PIK3CB) plays a role in regulating the formation and stability of alpha-2B-beta-3 integrin adhesion bonds, which are necessary for shear force-induced platelet activation. In animal model of prostate tumor formation induced by the tumor suppressor PTEN loss, ablation of p110 beta impedes tumorigenesis with a concomitant diminution of AKT phosphorylation.
• Source: Insect cells
• Species: Human
• Tag: His
• Form: 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
• Bio-activity: 510 nmol/min/mg
• Molecular Mass: p110 beta ~111 kDa and p85 alpha ~86 kDa
• Purity: >80%
• Applications: Kinase Assay
• Stability: 1 year at -70 centigrade from the date of shipment
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1μg/μl

Gene Information

• Gene Name: PIK3CB phosphoinositide-3-kinase, catalytic, beta polypeptide [ Homo sapiens ]
• Official Symbol: PIK3CB
• Synonyms: PIK3CB; phosphoinositide-3-kinase, catalytic, beta polypeptide; PIK3C1; phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta isoform; PI3K-beta; PtdIns-3-kinase p110; PI3-kinase subunit beta; PI3-kinase p110 subunit beta; ptdIns-3-kinase subunit beta; ptdIns-3-kinase subunit p110-beta; phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit beta; PI3K; P110BETA; PI3KBETA; MGC133043; DKFZp779K1237
• Gene ID: 5291
• mRNA Refseq: NM_006219
• Protein Refseq: NP_006210
• MIM: 602925
• UniProt ID: P42338
• Chromosome Location: 3q21-qter
• Pathway: 3-phosphoinositide biosynthesis, organism-specific biosystem; 3-phosphoinositide biosynthesis, conserved biosystem; Acute myeloid leukemia, organism-specific biosystem; Acute myeloid leukemia, conserved biosystem; Adaptive Immune System, organism-specific biosystem; Aldosterone-regulated sodium reabsorption, organism-specific biosystem; Aldosterone-regulated sodium reabsorption, conserved biosystem
• Function: 1-phosphatidylinositol-3-kinase activity; ATP binding; binding; insulin receptor substrate binding; nucleotide binding; phosphatidylinositol 3-kinase activity; phosphatidylinositol 3-kinase activity; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; phosphotransferase activity, alcohol group as acceptor