Recombinant Human PIK3CB 293 Cell Lysate

• Cat.No.: PIK3CB-3188HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for phosphoinositide-3-kinase, catalytic, beta polypeptide (PIK3CB) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: PIK3CB phosphoinositide-3-kinase, catalytic, beta polypeptide [ Homo sapiens ]
• Official Symbol: PIK3CB
• Synonyms: PIK3CB; phosphoinositide-3-kinase, catalytic, beta polypeptide; PIK3C1; phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta isoform; PI3K-beta; PtdIns-3-kinase p110; PI3-kinase subunit beta; PI3-kinase p110 subunit beta; ptdIns-3-kinase subunit beta; ptdIns-3-kinase subunit p110-beta; phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit beta; PI3K; P110BETA; PI3KBETA; MGC133043; DKFZp779K1237
• Gene ID: 5291
• mRNA Refseq: NM_001256045
• Protein Refseq: NP_001242974
• MIM: 602925
• UniProt ID: P42338
• Chromosome Location: 3q21-qter
• Pathway: 3-phosphoinositide biosynthesis, organism-specific biosystem; 3-phosphoinositide biosynthesis, conserved biosystem; Acute myeloid leukemia, organism-specific biosystem; Acute myeloid leukemia, conserved biosystem; Adaptive Immune System, organism-specific biosystem; Aldosterone-regulated sodium reabsorption, organism-specific biosystem; Aldosterone-regulated sodium reabsorption, conserved biosystem
• Function: 1-phosphatidylinositol-3-kinase activity; ATP binding; binding; insulin receptor substrate binding; nucleotide binding; phosphatidylinositol 3-kinase activity; phosphatidylinositol 3-kinase activity; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; phosphotransferase activity, alcohol group as acceptor