Active Recombinant Human NECTIN2 protein(Met1-Leu360), His&Avi-tagged, Biotinylated

• Cat.No.: NECTIN2-3254H
• Product Overview: Biotinylated Recombinant Human NECTIN2 (NP_002847.1)(Met1-Leu360) was expressed in HEK293 with a C-terminal polyhistidine tag followed by an AVI tag.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: Met1-Leu360
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Immobilized Human PVRIG hFc at 2 μg/mL (100 μL/well) can bind Human Nectin-2, Biotinylated, the EC50 of Human Nectin-2, Biotinylated is 0.6-1.5 μg/mL.
• Molecular Mass: The recombinant human NECTIN2 consists of 355 amino acids and predicts a molecular mass of 38.80 kDa. It migrates as an approximately 47.72 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.