Recombinant Human NECTIN2 protein(Met1-Leu360), hFc&Avi-tagged, Biotinylated

• Cat.No.: NECTIN2-0632H
• Product Overview: Recombinant Human NECTIN2 (NP_002847.1)(Met1-Leu360) was expressed in HEK293 with a c-terminal AVI tagged Fc region of Human IgG1 at the C-terminus (Fc-AVI). The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&Fc
• Protein Length: Met1-Leu360
• Conjugation/Label: Biotin
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized Human PVRIG hFc at 2 μg/mL (100 μL/well) can bind Human Nectin-2 (ECD, hFc & AVI Tag), Biotinylated, the EC50 of Human Nectin-2 (ECD, hFc & AVI Tag), Biotinylated is 10-60 ng/mL.
• Molecular Mass: The recombinant human NECTIN2 consists of 582 amino acids and predicts a molecular mass of 64.08 kDa. It migrates as an approximately 75.99 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.