Recombinant Human NECTIN2 protein, hFc-tagged

• Cat.No.: NECTIN2-233H
• Product Overview: Recombinant Human NECTIN2 protein(Q92692-2)(Gln32-Leu360), fused with C-terminal hFc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: Gln32-Leu360
• Tag: C-hFc
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human PVRIG, mFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human Nectin-2, hFc Tag with the EC50 of 24.1ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 62.3 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.