Active Recombinant Human MMP9 protein, His-tagged

• Cat.No.: MMP9-4893H
• Product Overview: Recombinant Human MMP9(Ala20-Asp707) fused with a C-terminal 8 His-tag and a linker sequence was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 20-707 a.a.
• Predicted N Terminal: Ala
• Form: 0.22 µm filtered protein solution is in TCN (25 mM TRIS, 10 mM CaCl2, 150 mM NaCl, pH 7.5).
• Bio-activity: Human MMP-9 cleaves the peptide substrate Mca-PLGL-Dpa-AR-NH2 with an activity above 1000 pmol/min/µg.
• Molecular Mass: This 722 amino acid recombinant protein has a predicted molecular mass of approximately 80 kD. The protein migrates at about 92 kD in DTT-reducing conditions and 250 kD in non-reducing conditions by SDS-PAGE.
• Endotoxin: Less than 1.0 EU per µg of protein as determine by the LAL method.
• Purity: >95%, as determined by Coomassie stained SDS-PAGE.
• Storage: Unopened vial can be stored at -70 centigrade for six months. For maximum results, quick spin vial prior to opening. Avoid repeated freeze/thaw cycles.

Gene Information

• Gene Name: MMP9 matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) [ Homo sapiens ]
• Official Symbol: MMP9
• Synonyms: MMP9; matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); CLG4B, matrix metalloproteinase 9 (gelatinase B, 92kD gelatinase, 92kD type IV collagenase) , matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); matrix metalloproteinase-9; 92 kDa gelatinase; type V collagenase; macrophage gelatinase; 92 kDa type IV collagenase; matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); GELB; CLG4B; MMP-9; MANDP2
• Gene ID: 4318
• mRNA Refseq: NM_004994
• Protein Refseq: NP_004985
• MIM: 120361
• UniProt ID: P14780
• Chromosome Location: 20q12-q13
• Pathway: Activation of Matrix Metalloproteinases, organism-specific biosystem; Bladder cancer, organism-specific biosystem; Bladder cancer, conserved biosystem; CXCR4-mediated signaling events, organism-specific biosystem; Degradation of the extracellular matrix, organism-specific biosystem
• Function: collagen binding; fibronectin binding; metal ion binding; metalloendopeptidase activity; peptidase activity; protein binding; protein complex binding; zinc ion binding