Active Recombinant Human KLK7

• Cat.No.: KLK7-894H
• Product Overview: Recombinant Human KLK7 /Kallikrein-7 Protein (rhKallikrein-7 /KLK7) Glu 23 - Arg 253 was produced in human 293 cells (HEK293).

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Protein Length: 23-253 a.a.
• Description: Kallikrein-7 (KLK7) is also known as kallikrein-related peptidase 7, Stratum corneum chymotryptic enzyme, Serine protease 6, KLK7, and PRSS6, is a secreted protein which belongs to the peptidase S1 family and Kallikrein subfamily. KLK7 is secreted as an inactive zymogen in the stratum granulosum layer of the epidermis, requiring proteolytic cleavage of the short N-terminal pro-region to liberate activated enzyme. This may be performed by KLK5 or matriptase, which are in vitro activators of KLK7. Once active, KLK7 is able to cleave desmocollin and corneodesmosin. KLK7 activity is regulated by a number of endogenous protein inhibitors including LEKTI, SPINK6, elafin and alpha-2-Macroglobulin-like 1. Both Zn2+ and Cu2+ ions are also able to inhibit KLK7. Dysregulation of KLK7 has been linked to several skin disorders, and overexpression of KLK7 may provide a route for metastasis in several cancers.
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH 7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK (Dnp) NH2. The specific activity is >130 pmoles / min / μg.
• Molecular Mass: rhKallikrein-7 /KLK7 is fused with polyhistidine tag at the C-terminus, and has a calculated MW of 26.1 kDa. The predicted N-terminus is Glu 23. DTT-reduced Protein migrates as 29-33 kDa in SDS-PAGE due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg of the rhKallikrein-7 /KLK7 by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).
• Reconstitution: See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Gene Information

• Gene Name: KLK7 kallikrein-related peptidase 7 [ Homo sapiens ]
• Official Symbol: KLK7
• Synonyms: KLK7; kallikrein-related peptidase 7; SCCE; PRSS6; kallikrein-7; hK7; Hscce; signal protein; serine protease 6; protease, serine, 6; stratum corneum chymotryptic enzyme; kallikrein 7 (chymotryptic, stratum corneum); EC 3.4.21.117
• Gene ID: 5650
• mRNA Refseq: NM_005046
• Protein Refseq: NP_005037
• MIM: 604438
• UniProt ID: P49862
• Chromosome Location: 19q13.41
• Pathway: Degradation of the extracellular matrix; Extracellular matrix organization
• Function: serine-type endopeptidase activity; serine-type peptidase activity