Active Recombinant Human Kallikrein-Related Peptidase 12, His-tagged

• Cat.No.: KLK12-1977H
• Product Overview: RecombinantHuman KLK12 protein was expressed in Murine myeloma cellline. Ala18-Asn248,with a C-terminal 10-His tag.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: His
• ProteinLength: 18-248 a.a.
• Description: Human tissue KLK12, encoded bythe KLK12 gene, is a secreted serine protease that belongs to the humantissue kallikrein family. It is present in many tissues, such as salivarygland, stomach and breast. KLK12 displays trypsinlike enzymatic activity. Thisactivity can be inhibited by Serpin F2. The physiological functions of KLK12still remain unclear. Its expression is modulated by steroid hormones and isdown regulated in breast cancer. Human KLK12 has three splice variantsresulting from alternative splicing of the 3' end.
• Form: Supplied as a 0.2 μmfiltered solution in MES, NaCl and Glycerol.
• N-terminalSequence: Ala18
• MolecularWeight: 26 kDa
• Activity: Measuredby its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC. Thespecific activity is > 4,000 pmoles/min/μg, as measured under thedescribed conditions.
• Endotoxin Level: <1.0EU per 1μg of the protein by the LAL method.
• SDS-PAGE: 37 kDa,reducing conditions.
• Purity: >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Use amanual defrost freezer and avoid repeated freezethaw cycles. 6 monthsfrom date of receipt, -20 to -70°C as supplied. 3 months,-20to-70°C under sterile conditions after opening.

Gene Information

• Gene Name: KLK12 kallikrein-relatedpeptidase 12 [ Homo sapiens ]
• Official Symbol: KLK12
• Synonyms: KLK12;kallikrein-related peptidase 12; KLKL5; KLK-L5; kallikrein-12; kallikrein12; kallikrein-like protein 5
• Gene ID: 43849
• mRNA Refseq: NM_019598
• Protein Refseq: NP_062544
• MIM: 605539
• UniProt ID: Q9UKR0
• Chromosome Location: 19q13.33
• Function: catalytic activity;peptidase activity; serine-type endopeptidase activity; serine-type peptidaseactivity