Active Recombinant Human ITGA6&ITGB1 protein, His-tagged

• Cat.No.: ITGA6&ITGB1-0306H
• Product Overview: Recombinant Human ITGA6&ITGB1 protein(Phe24-Gly1012(ITGA6)&Gln21-Asp728(ITGB1)), fused with His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 24-1012;21-728 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.
• Bio-activity: Immobilized Human ITGA6&ITGB1 Heterodimer Protein, His Tag&Tag Free at 5 μg/mL (100 μL/well) can bind Human Laminin 511 E8, Fc Tag with a linear range of 0.1-8 ng/mL.
• Molecular Mass: The non-reducing (NR) protein migrates as 140-150 kDa (ITGA6) and 100-118 kDa (ITGB1) respectively due to glycosylation.
• Proteinlength: Phe24-Gly1012(ITGA6)&Gln21-Asp728(ITGB1)
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: ITGA6 integrin, alpha 6 [ Homo sapiens ]
• Official Symbol: ITGA6
• Synonyms: ITGA6; integrin, alpha 6; integrin alpha-6; CD49f; integrin alpha6B; CD49 antigen-like family member F; VLA-6; ITGA6B; FLJ18737; DKFZp686J01244
• Gene ID: 3655
• mRNA Refseq: NM_000210
• Protein Refseq: NP_000201
• MIM: 147556
• UniProt ID: P23229