Recombinant Human ITGA6 & ITGB1 Protein, His-Avi-tagged, Biotinylated

• Cat.No.: ITGA6 & ITGB1-609H
• Product Overview: Biotinylated Human ITGA6 & ITGB1 Heterodimer Protein is expressed from HEK293 cells. It contains AA Phe 24 - Gly 1012 (ITGA6) & Gln 21 - Asp 728 (ITGB1) (Accession # P23229-2 (ITGA6) & P05556-1 (ITGB1)). Subunit ITGA6 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and an Avi tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His&Avi
• ProteinLength: 24-1012;21-728 a.a.
• Predicted N Terminal: Phe 24 (ITGA6) & Gln 21 (ITGB1)
• Form: Lyophilized from 0.22 um filtered solution in 50 mM Tris, 150 mM NaCl, pH 7.5, 10% trehalose.
• Molecular Mass: Human ITGA6 & ITGB1 Heterodimer Protein, produced by co-expression of ITGA6 and ITGB1, has a calculated MW of 117.0 kDa (ITGA6) and 84.0 kDa (ITGB1).
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Characteristic: Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Official Symbol: ITGA6; ITGB1
• Synonyms: Integrin alpha 6 beta 1, ITGA6 & ITGB1