Active Recombinant Human IL4,His-tagged

• Cat.No.: IL4-8863H
• Product Overview: Recombinant Human IL4 was produced in the endosperm tissue of barley grain (Hordeum vulgare).

Specification

• Species: Human
• Source: Hordeum Vulgare
• Tag: Non
• Description: IL-4 (Interleukin 4, B cell stimulatory factor) is a pleiotropic cytokine produced by activated T cells, mast cells and basophils. IL-4 has many different functions including promoting proliferation and differentiation of immunologically competent cells. One of its main functions is regulating differentiation of naive CD4+ T cells into helper Th2 cells, the other main function is regulating B cells IgE and IgG1 production. Human IL-4 shows 50% homology with mouse IL-4, but its activities are species-specific and human IL-4 shows no activity on murine and rat cells.
• Predicted N Terminal: His
• Form: Sterile filtered through a 0.2 μm filter. Lyophilized from PBS, pH7,2
• Bio-activity: The bioactivity of recombinant human IL-4 was determined by its dose-dependent effects on the proliferation of TF-1 cells. The ED50 value for this effect is typically = 0.2 ng/ml, corresponding to a specific activity of = 5 x 10e6 units/mg.
• Molecular Mass: Recombinant human IL-4 contains 130 amino acids and a 16 a.a. Histidine-based tag for a total length of 146 a.a. and has a predicted molecular mass of 17.2 kDa. The recombinant protein migrates as two bands with an apparent molecular mass of 23 kDa and 24 kDa in SDS-PAGE.
• Endotoxin: Endotoxin level is less than 0.05EU/μg as measured by turbidimetric kinetic assay.
• Purity: >95% by SDS-PAGE gel analysis.
• Storage: The lyophilized protein is stable at room temperature for few weeks. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile water to a concentration of no less than 100 μg/ml. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Gene Information

• Gene Name: IL4 interleukin 4 [ Homo sapiens ]
• Official Symbol: IL4
• Synonyms: IL4; interleukin 4; interleukin-4; B cell growth factor 1; B_cell stimulatory factor 1; BCGF 1; BCGF1; BSF1; IL 4; lymphocyte stimulatory factor 1; MGC79402; binetrakin; pitrakinra; IL-4; BSF-1; BCGF-1
• Gene ID: 3565
• mRNA Refseq: NM_000589
• Protein Refseq: NP_000580
• MIM: 147780
• UniProt ID: P05112
• Chromosome Location: 5q23-q31
• Pathway: Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Asthma, organism-specific biosystem; Asthma, conserved biosystem; Autoimmune thyroid disease, organism-specific biosystem; Autoimmune thyroid disease, conserved biosystem; CD40/CD40L signaling, organism-specific biosystem
• Function: cytokine activity; growth factor activity; interleukin-4 receptor binding; protein binding