Active Recombinant Bovine Interleukin 4

• Cat.No.: IL4-240B
• Product Overview: Recombinant Bovine IL-4 was expressed inYeast. MW= 12.6 kDa (112 aa).

Specification

• Species: Bovine
• Source: Yeast
• Tag: Non
• Description: Interleukin-4, abbreviated IL-4, is a cytokine that induces differentiation of naive helper T cells (Th0 cells) to Th2 cells. Upon activation by IL-4, Th2 cells subsequently produce additional IL-4. The cell that initially produces IL-4, thus inducing Th0 differentiation, has not been identified, but recent studies suggest that basophils may be the effector cell. Its receptor is the Interleukin-4 receptor.
• Formulation/ Reconstitution: Lyophilized. Reconstitute with sterile phosphate-buffered saline containing at least 0.1% carrier protein.
• Purification: Ion-exchange chromatography.
• Purity: >95% as visualized by SDS-PAGE analysis.
• Amino Acid Sequence: GHKCDITLAE IIKTLNILTT RKNSCMELPV ADVFAAPKNT TEKETFCRVG IELRRIYRSH TCLNKFLGGL DRNLNSLASK TCSVNEAKTS TSTLKDLLER LKTIMKEKYS KC.
• Bioactivity: The biological activity of recombinant bovine IL-4 was measured in a cell proliferation assay using the human TF-1 cell line. The ED50 for this effect is typically 1.5 - 2 ng/mL.
• Stability And Storage: Stable for up to twelve months from date of receipt at -20°C. Stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. Avoid repeated freeze/thaw cycles.

Gene Information

• Gene Name: IL4 interleukin 4 [ Bos taurus ]
• Synonyms: interleukin 4; IL4; IL-4; interleukin-4; BSF-1; B-cell stimulatory factor 1; lymphocyte stimulatory factor 1
• Gene ID: 280824
• mRNA Refseq: NM_173921.2
• Protein Refseq: NP_776346.1
• UniProt ID: P30367
• Chromosome Location: 7q15-q21
• Pathway: Allograft rejection; Asthma; Autoimmune thyroid disease; Cytokine-cytokine receptor interaction; Fc epsilon RI signaling pathway; Hematopoietic cell lineage; Intestinal immune network for IgA production; Jak-STAT signaling pathway; T cell receptor signaling pathway
• Function: cytokine activity; growth factor activity; interleukin-4 receptor binding; protein binding