Active Recombinant Human IL1B Protein

• Cat.No.: IL1B-059H
• Product Overview: The mature recombinant human IL1beta protein consists of 153 amino acids (117-269) without tag was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Protein Length: 117-269 a.a.
• Description: The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. Similarly, IL-1B has been implicated in human osteoarthritis pathogenesis. Patients with severe Coronavirus Disease 2019 (COVID-19) present elevated levels of pro-inflammatory cytokines such as IL-1B in bronchial alveolar lavage fluid samples. The lung damage induced by the Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is to a large extent, a result of the inflammatory response promoted by cytokines such as IL-1B. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.
• Bio-activity: Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 for this effect is <12 pg/mL.
• Molecular Mass: 17 kDa
• Product-Related Proteins: 117-269
• Endotoxin: < 0.1 EU/μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: At -80 centigrade. Avoid repeated freeze-thaw cycles.
• Storage Buffer: PBS

Gene Information

• Gene Name: IL1B interleukin 1 beta [ Homo sapiens (human) ]
• Official Symbol: IL1B
• Synonyms: IL1B; interleukin 1 beta; IL-1; IL1F2; IL1beta; IL1-BETA; interleukin-1 beta; IL-1 beta; catabolin; interleukin 1beta; preinterleukin 1 beta; pro-interleukin-1-beta
• Gene ID: 3553
• mRNA Refseq: NM_000576
• Protein Refseq: NP_000567
• MIM: 147720
• UniProt ID: P01584