Active Recombinant Human IgG1Fc protein

• Cat.No.: IgG1Fc-07H
• Product Overview: Recombinant Human IgG1Fc protein(Pro 100 - Lys 330) was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• ProteinLength: 100-330 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Immobilized Human IgG1 Fc (C103S, D239E, L241E), Tag Free at 5 μg/mL (100 μL/well) can bind Biotinylated Human CD64, His,Avitag with a linear range of 0.3-2 ng/mL.Immobilized Human IgG1 Fc (C103S, D239E, L241E), Tag Free at 5 μg/mL (100 μL/well) can bind Biotinylated Human CD16a (V176), Avitag,His Tag with a linear range of 0.02-0.625 μg/mL.
• Molecular Mass: This protein carries no "tag"The protein has a calculated MW of 26 kDa. The protein migrates as 31-33 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Pro100-Lys330
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE. >95% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.