Active Recombinant Mouse IgG1Fc protein

• Cat.No.: IgG1Fc-10M
• Product Overview: Recombinant Mouse IgG1Fc protein(Val 111 - Lys 324) was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Non
• ProteinLength: 111-324 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in 51 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Loaded Mouse FCGRT&B2M Heterodimer Protein, His Tag on NTA Biosensor, can bind Mouse IgG Fc, Tag Free with an affinity constant of 17.9 nM as determined in BLI assay (ForteBio Octet Red96e).
• Molecular Mass: This protein carries no "tag".The protein has a calculated MW of 24.3 kDa. The protein migrates as 28-30 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Val111-Lys324
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.>95% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.