Active Recombinant Human IGF1 (26-95aa)

• Cat.No.: IGF1-026E
• Product Overview: Recombinant Human IGF1 (Gly26 ­ Ala95) was expressed in E. coli and produced using non­animal reagent in an animal­free laboratory.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: Insulin­like growth factor I, also known as somatomedin C, is the dominant effector of growth hormone and is structurally homologous to proinsulin. Human IGF­I is synthesized as two precursor isoforms with N­ and alternate C­terminal propeptides . IGF­I induces the proliferation, migration, and differentiation of a wide variety of cell types during development and postnatally . IGF­I regulates glucose and fatty acid metabolism, steroid hormone activity, and cartilage and bone metabolism . It plays an important role in muscle regeneration and tumor progression. IGF ­I binds IGF­I R, IGF­II R, and the insulin receptor, although its effects are mediated primarily by IGF­I R. IGF­I association with IGF binding proteins increases its plasma half­life and modulates its interactions with receptors.
• N­terminal Sequence Analysis: Gly26
• Molecular Mass: 7.5 kDa
• Activity: Measured in a serum­free cell proliferation assay using MCF­7 human breast denocarcinoma cells. The ED50 for this effect is typically 0.3 ­ 1.5 ng/mL.
• Endotoxin Leve: <1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >97%, by SDS­PAGE under reducing conditions and visualized by silver stain.
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS. See Certificate of Analysis for details.
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
• Storage: Use a manual defrost freezer and avoid repeated freeze­thaw cycles. 1. 12 months from date of receipt, ­20 to ­70 °C as supplied. 2. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3. 3 months, ­20 to ­70 °C under sterile conditions after reconstitution.
• Unitprot ID: Q13429

Gene Information

• Gene Name: IGF1 insulin-like growth factor 1 (somatomedin C) [ Homo sapiens ]
• Official Symbol: IGF1
• Synonyms: IGF1; insulin-like growth factor 1 (somatomedin C); IGFI; IGF-I; IGF1A; insulin-like growth factor 1; MGF; IGF-IA; IGF-IB; somatomedin-C; mechano growth factor; insulin-like growth factor I; insulin-like growth factor IA; insulin-like growth factor IB
• Gene ID: 3479
• mRNA Refseq: NM_001111283
• Protein Refseq: NP_001104753
• MIM: 147440
• Chromosome Location: 12q23.2
• Pathway: Androgen Receptor Signaling Pathway; Aldosterone-regulated sodium reabsorption; Androgen Receptor Signaling Pathway; Diabetes pathways; Hypertrophic cardiomyopathy (HCM); Id Signaling Pathway; Pathways in cancer; mTOR signaling pathway; p53 signaling pathway
• Function: growth factor activity; hormone activity; insulin receptor binding; insulin-like growth factor receptor binding; integrin binding; protein binding