Active Recombinant Human HSPA4 Protein

• Cat.No.: HSPA4-012H
• Product Overview: Recombinant Human HSPA4 protein (2-641) without tag was espressed in insect cell.

Specification

• Species: Human
• Source: Insect Cells
• Protein Length: 2-641
• Description: HSPA4 heat shock protein family A (Hsp70) member 4 are abundant and stress-inducible 70 kDa molecular chaperone proteinsencoded by a highly conserved, multigene family. They are monomeric proteins that can be divided into two functional domains: an N-terminal ATPase domain and a substrate binding domain that contains a highly conserved EEVD motif at its C-terminus. Hsp70s are found in the cytosol, nuclei, endoplasmic reticulum, mitochondria, and chloroplasts of eukaryotes, as well as in bacteria. They function as molecular chaperones that assist in a wide range of cellular processes, including refolding of aggregated or misfolded proteins, co- and post-translational folding and assembly of nascent peptides, membrane translocation of secretory and organellar proteins, controlling activity of regulatory nuclear receptors, kinases and transcription factors, as well as acting cooperatively with the Hsp90 chaperone system in eukaryotes. The HSPA4 chaperone cycle is ATP-dependent and initiated by transient interaction of the HSPA4 substrate binding domain with hydrophobic regions within a peptide or protein. It consists of an alteration between the low-affinity ATP-bound state with fast rates of substrate exchange and the high-affinity ADP bound state with slow rates of substrate exchange. HSPA4 are subject to a variety of post-translational modifications and their expression is upregulated under conditions of cellular stress and in a variety of disease states.
• Form: 50 mM HEPES, pH 8.0, 150 mM sodium chloride, 1 mM DTT, and 10% glycerol
• Bio-activity: ATPase activity confirmed by ADP detection assay
• Molecular Mass: 71.7 kDa
• Purity: ≥75% estimated by SDS-PAGE
• Stability: ≥ 1 year
• Storage: Store at -80 centigrade

Gene Information

• Gene Name: HSPA4 heat shock protein family A [ Homo sapiens (human) ]
• Official Symbol: HSPA4
• Synonyms: HSPA4; heat shock protein family A; RY; APG-2; HSPH2; hsp70; hsp70RY; HEL-S-5a; HS24/P52; heat shock 70 kDa protein 4; epididymis secretory sperm binding protein Li 5a; heat shock 70-related protein APG-2; heat shock 70kD protein 4; heat shock 70kDa protein 4; heat shock protein, 110 kDa; hsp70 RY
• Gene ID: 3308
• mRNA Refseq: NM_002154
• Protein Refseq: NP_002145
• MIM: 601113
• UniProt ID: P34932