Active Recombinant Human GDF11

• Cat.No.: GDF11-1078H
• Product Overview: Recombinant Human GDF11 (Accession # O95390) Asn299-Ser407, fused with an N-terminal Met, was produced in E. coli.

Specification

• Source: E. coli
• Species: Human
• Predicted N Terminal: Met-Asn299-Leu-Gly-Leu-Asp-(Cys)-Asp-Glu-His
• Form: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
• Bio-activity: Measured by its ability to induce hemoglobin expression in K562 human chronic myelogenous leukemia cells. Schwall, R.H. et al. (1991) Method Enzymol. 198:340. The ED50
• Molecular Mass: Recombinant Human GDF11, Met-tagged has a calculated MW of 12.6 kDa. In SDS-PAGE migrates as 13 kDa, reducing conditions.
• Protein length: Asn299-Ser407
• Purity: Assessed by mass spectrometry, and is >95% pure by SDS-PAGE under reducing conditions visualized by silver stain (see below).
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 100 µg/mL in 4 mM HCl containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: GDF11 growth differentiation factor 11 [ Homo sapiens ]
• Official Symbol: GDF11
• Synonyms: GDF11; growth differentiation factor 11; growth/differentiation factor 11; BMP 11; GDF-11; bone morphogenetic protein 11; BMP11; BMP-11
• Gene ID: 10220
• mRNA Refseq: NM_005811
• Protein Refseq: NP_005802
• MIM: 603936
• UniProt ID: O95390
• Chromosome Location: 12q13.13