Active Recombinant Human FMOD protein, His-tagged

• Cat.No.: FMOD-82H
• Product Overview: Recombinant Human FMOD protein (Asp75-­Ile376), fused to His-tag at N-terminus, was expressed in mouse myeloma cell line, NS0-­derived.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: His
• Form: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Bio-activity: Measured by its binding ability in a functional ELISA. When Bovine Collagen-I is immobilized at 10 μg/mL, 100 μL/well, the concentration of Recombinant Human Fibromodulin/FMOD that produces 50% of the optimal binding response is 1-6 μg/mL
• Molecular Mass: 36 kDa
• Protein length: 302
• Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 200 μg/mL in PBS.

Gene Information

• Gene Name: FMOD
• Official Symbol: FMOD
• Synonyms: FM; SLRR2E
• Gene ID: 2331
• mRNA Refseq: NM_002023.5
• Protein Refseq: NP_002014.2
• MIM: 600245
• UniProt ID: Q06828