Active Recombinant Human FGF21 Protein (His29-Ser209), C-His tagged, Animal-free, Carrier-free

• Cat.No.: FGF21-117H
• Product Overview: Recombinant Human FGF21 Protein (His29-Ser209) with C-His tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: His29-Ser209
• Description: Fibroblast Growth Factors-21 (FGF-21) is a 22.3 kDa member of the fibroblast Growth Factors with 209 amino acid residues. FGF-21 is expressed from liver and cardiomyocytes. FGF-21 is a key protein that regulates important metabolic pathways, and modulates cellular function, metabolism, and senescence. It can stimulate glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1 /GLUT1 expression.
• Form: Lyophilized
• Bio-activity: Measure by its ability to induce proliferation in BaF3 cells transfected with human FGFRIIIc. The ED50 for this effect is < 0.4 μg/mL.
• Molecular Mass: The protein has a calculated MW of 20.35 kDa.
  The protein migrates as 25 kDa under reducing condition (SDS-PAGE analysis).
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: >98% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 8.0.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: FGF21 fibroblast growth factor 21 [ Homo sapiens (human) ]
• Official Symbol: FGF21
• Synonyms: FGF21; fibroblast growth factor 21; FGF-21
• Gene ID: 26291
• mRNA Refseq: NM_019113
• Protein Refseq: NP_061986
• MIM: 609436
• UniProt ID: Q9NSA1