Active Recombinant Human EGFL6 Protein (Asn22-Asp553), His-FLAG-tagged

• Cat.No.: EGFL6-447H
• Product Overview: Recombinant Human EGFL6 Protein (NP_056322.2) (Asn22-Asp553) was produced by Baculovirus-Insect Cells expression system. This protein was expressed with the flag tag at the N-terminus and the histidine tag at the C-terminus.

Specification

• Description: EGFL6, also known as EGF-L6, belongs to the epidermal growth factor (EGF) repeat superfamily. Members of this superfamily are characterized by the presence of EGF-like repeats and are often involved in the regulation of cell cycle, proliferation, and developmental processes. EGFL6 contains a signal peptide, suggesting that it is secreted; an EGF repeat region consisting of 4 complete EGF-like repeats and 1 partial EGF-like repeat, 3 of which have a calcium-binding consensus sequence; an arg-gly-asp integrin association motif; and a MAM domain, which is believed to have an adhesive function. EGFL6 gene is expressed early during development, and its expression has been detected in lung and meningioma tumors.
• Source: Baculovirus-Insect Cells
• Species: Human
• Tag: His&Flag
• Predicted N Terminal: Met
• Form: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 20 % glycerol, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells.When 5 × 10E4 cells/well areadded to CD4-coated plates (1.25μg/mL and 100μL/well),approximately 40%-60% will adhere specifically after 30 minutes at 37 centigrade.
• Molecular Mass: The recombinant human EGFL6 consists of 551 amino acids and has a calculated molecular mass of 61.5 kDa.
• Protein length: Asn22-Asp553
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 65 % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 centigrade to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4℃ before opening to recover the entire contents.
• Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
  Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Gene Information

• Gene Name: EGFL6 EGF like domain multiple 6 [ Homo sapiens (human) ]
• Official Symbol: EGFL6
• Synonyms: W80; MAEG
• Gene ID: 25975
• mRNA Refseq: NM_015507
• Protein Refseq: NP_056322
• MIM: 300239
• UniProt ID: Q8IUX8