Active Recombinant Human CYP2C9 Protein

• Cat.No.: CYP2C9-22H
• Product Overview: Recombinant human CYP2C9 and human CYP-reductase without tag was coexpressed in Escherichia coli.
  Amount: 0.5 nmol cytochrome P450

Specification

• Description: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by rifampin. The enzyme is known to metabolize many xenobiotics, including phenytoin, tolbutamide, ibuprofen and S-warfarin. Studies identifying individuals who are poor metabolizers of phenytoin and tolbutamide suggest that this gene is polymorphic. The gene is located within a cluster of cytochrome P450 genes on chromosome 10q24.
• Source: E. coli
• Species: Human
• Bio-activity: Cytochrome c Reductase Activity: 84 nmol/min/mg protein
  P450 Activity Data(Unless otherwise stated, all assays are carried out at 37 centigrade in 50 mM potassium phosphate buffer (pH 7.4) containing 5 mM magnesium chloride and an NADPH generating system [5 mM glucose 6-phosphate, 1 mM NADP+, 1 U/ml glucose 6-phosphate dehydrogenase])
  Activity: Diclofenac 4’-hydroxylase
  Linear with time up to: 12 min (Determined at 2.5 µM substrate, using a P450 concentration of 10 pmol/ml)
  Linear with P450 up to: 25 pmol/ml (Determined at 2.5 µM substrate, using an incubation time of 5 min)
  Vmax: 14 pmol/min/pmol CYP
  Km: 3.2 µM
• Usage: For laboratory (research) purposes only.
• Storage: Store at -80 centigrade. Avoid frequent temperature changes. Thaw on ice.
• Concentration: P450 Concentration: 1.0 nmol/ml
  Protein Concentration: 10.0 mg/ml
  Specific P450 Content: 100 pmol/mg protein
• Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA

Gene Information

• Gene Name: CYP2C9 cytochrome P450 family 2 subfamily C member 9 [ Homo sapiens (human) ]
• Official Symbol: CYP2C9
• Synonyms: CYP2C9; cytochrome P450 family 2 subfamily C member 9; CPC9; CYP2C; CYP2C10; CYPIIC9; P450IIC9; cytochrome P450 2C9; cytochrome P-450 S-mephenytoin 4-hydroxylase; cytochrome P-450MP; cytochrome P450 PB-1; cytochrome P450, family 2, subfamily C, polypeptide 9; flavoprotein-linked monooxygenase; microsomal monooxygenase; xenobiotic monooxygenase; EC 1.14.14.1; EC 1.14.14.51; EC 1.14.14.52; EC 1.14.14.53
• Gene ID: 1559
• mRNA Refseq: NM_000771
• Protein Refseq: NP_000762
• MIM: 601130
• UniProt ID: P11712