Active Recombinant Human CSF3 Protein

• Cat.No.: CSF3-178H
• Product Overview: Recombinant human GCSF isoform b (NP_757373.1) (Ala30-Pro204) was expressed.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Protein Length: 30-204 a.a.
• Predicted N Terminal: Ala 30
• Form: Lyophilized from sterile sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
• Bio-activity: Measured in a cell proliferation assay using NFS-60 mouse myelogenous leukemia lymphoblast cells.The ED50 for this effect is typically 0.04-0.2 ng/mL.
• Molecular Mass: The recombinant human GCSFb consists of 175 amino acids and has a calculated molecular mass of 18.7 kDa. It migrates as an approximately 18 kDa protein in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >90 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: CSF3 colony stimulating factor 3 (granulocyte) [ Homo sapiens ]
• Official Symbol: CSF3
• Synonyms: CSF3; colony stimulating factor 3 (granulocyte); C17orf33, chromosome 17 open reading frame 33 , G CSF, GCSF; granulocyte colony-stimulating factor; filgrastim; granulocyte colony stimulating factor; lenograstim; MGC45931; pluripoietin; GCSF; CSF3OS; C17orf33
• Gene ID: 1440
• mRNA Refseq: NM_000759
• Protein Refseq: NP_000750
• MIM: 138970
• UniProt ID: P09919
• Chromosome Location: 17q11.2-q12
• Pathway: Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Cytokines and Inflammatory Response, organism-specific biosystem; Hematopoietic cell lineage, organism-specific biosystem; Hematopoietic cell lineage, conserved biosystem; Jak-STAT signaling pathway, organism-specific biosystem; Jak-STAT signaling pathway, conserved biosystem