Active Recombinant Human CSF3 Protein, Pre-aliquoted

• Cat.No.: CSF3-10H
• Product Overview: Recombinant Human CSF3 Protein, Thr31 - Pro204, with an -N-terminal Met, was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Protein Length: Thr31-Pro204, with an N-terminal Met
• Description: This gene encodes a member of the IL-6 superfamily of cytokines. The encoded cytokine controls the production, differentiation, and function of granulocytes. Granulocytes are a type of white blood cell that are part of the innate immune response. A modified form of this protein is commonly administered to manage chemotherapy-induced neutropenia. Alternatively spliced transcript variants have been described for this gene.
• Bio-activity: Measured in a cell proliferation assay using NFS‑60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is 10-60 pg/mL.
• Molecular Mass: Predicted Molecular Mass:18.8 kDa
• N-terminal Sequence Analysis: Met
• Endotoxin: < 0.01 EU/μg of the protein by the LAL method.
• Purity: > 97% by SDS-PAGE under reducing conditions and visualized by silver stain.
• Stability: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt at room temperature.
  • 12 months from date of receipt at 2-8 centigrade as supplied.
  • 1 month at 2-8 centigrade under sterile conditions after reconstitution.
  • 3 months at -20 to -80 centigrade under sterile conditions after reconstitution.
• Reconstitution: For a stock solution, reconstitute at 50 μg/mL in sterile PBS, or simply roll Pre-aliquoted directly into the cell culture medium for immediate use.
• Shipping: The product is shipped at ambient temperature.

Gene Information

• Gene Name: CSF3 colony stimulating factor 3 [ Homo sapiens (human) ]
• Official Symbol: CSF3
• Synonyms: CSF3; colony stimulating factor 3; GCSF; CSF3OS; C17orf33; granulocyte colony-stimulating factor; filgrastim; granulocyte-colony stimulating factor; lenograstim; pluripoietin
• Gene ID: 1440
• mRNA Refseq: NM_000759
• Protein Refseq: NP_000750
• MIM: 138970
• UniProt ID: P09919