Active Recombinant Human CSF1 Protein, Pre-aliquoted

• Cat.No.: CSF1-12H
• Product Overview: Recombinant Human CSF1 Protein, Glu33 - Ser190, with an N-terminal Met, was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Protein Length: Glu33-Ser190, with an N-terminal Met
• Description: The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of macrophages. The active form of the protein is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. The encoded protein may be involved in development of the placenta. Alternate splicing results in multiple transcript variants.
• Bio-activity: Measured in a cell proliferation assay using M‑NFS‑60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is 0.5-1.5 ng/mL.
• Molecular Mass: Predicted Molecular Mass:18.5 kDa (monomer)
• N-terminal Sequence Analysis: Met
• Endotoxin: < 0.10 EU/μg of the protein by the LAL method.
• Purity: > 97% by SDS-PAGE under reducing conditions and visualized by silver stain.
• Stability: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt at room temperature.
  • 12 months from date of receipt at 2-8 centigrade as supplied.
  • 1 month at 2-8 centigrade under sterile conditions after reconstitution.
  • 3 months at -20 to -80 centigrade under sterile conditions after reconstitution.
• Reconstitution: For a stock solution, reconstitute at 50-500 μg/mL in sterile PBS, or simply roll Pre-aliquoted directly into cell culture medium.
• Shipping: The product is shipped at ambient temperature.

Gene Information

• Gene Name: CSF1 colony stimulating factor 1 [ Homo sapiens (human) ]
• Official Symbol: CSF1
• Synonyms: CSF1; colony stimulating factor 1; MCSF; CSF-1; macrophage colony-stimulating factor 1; colony stimulating factor 1 (macrophage); lanimostim; macrophage colony stimulating factor 1; EC 2.7.10.1
• Gene ID: 1435
• mRNA Refseq: NM_172211
• Protein Refseq: NP_757350
• MIM: 120420
• UniProt ID: P07333