Active Recombinant Human CSF1 (33-190aa), Met tagged

• Cat.No.: CSF1-033E
• Product Overview: Recombinant Human CSF1 (Glu33-Ser190, with an N­terminal Met) was expressed in E. coli and produced using non­animal reagent in an animal­free laboratory.

Specification

• Cat. No.: CSF1-033E
• Description: M­CSF, also known as CSF­1, is a four-­α­-helical bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation. M­CSF is also essential for the survival and proliferation of osteoclast progenitors. M­CSF also primes and enhances macrophage killing of tumor cells and microorganisms, regulates the release of cytokines and other inflammatory modulators from macrophages, and stimulates pinocytosis . M­CSF increases during pregnancy to support implantation and growth of the decidua and placenta. Sources of M­CSF include fibroblasts, activated macrophages, endometrial secretory epithelium, bone marrow stromal cells and activated endothelial cells.
• Source: E. coli
• N­terminal Sequence Analysi: Met
• Molecular Mass: 18.5 kDa
• SDS­PAGE: 37 kDa, non­reducing conditions
• Activity: Measured in a cell proliferation assay using M­NFS­60 mouse myeloid cells. The ED50 for this effect is typically 0.5 ­ 1.5 ng/mL.
• Endotoxin Leve: <1.0 EU per 1μg of the protein by the LAL method.
• Purity: >97%, by SDS­PAGE under reducing conditions and visualized by silver stain.
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS. See Certificate of Analysis for details.
• Reconstitution: Reconstitute at 200 μg/mL in sterile PBS.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
• Storage: Use a manual defrost freezer and avoid repeated freeze­thaw cycles. 1. 12 months from date of receipt, ­20 to ­70 °C as supplied. 2. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3. 3 months, ­20 to ­70 °C under sterile conditions after reconstitution.
• Unitprot ID: P09603
• Tag: Non
• Protein length: 33-190 a.a.

Gene Information

• Gene Name: CSF1 colony stimulating factor 1 (macrophage) [ Homo sapiens ]
• Official Symbol: CSF1
• Synonyms: CSF1; colony stimulating factor 1; MCSF; MGC31930; macrophage colony-stimulating factor 1; CSF-1; lanimostim; macrophage colony stimulating factor
• Gene ID: 1435
• mRNA Refseq: NM_000757
• Protein Refseq: NP_000748
• MIM: 120420
• Chromosome Location: 1p21-p13
• Pathway: Cytokine-cytokine receptor interaction; Hematopoietic cell lineage; Osteoclast differentiation; Rheumatoid arthritis; Signaling events mediated by PTP1B; Signaling events mediated by TCPTP
• Function: cytokine activity; growth factor activity; macrophage colony-stimulating factor receptor binding; protein homodimerization activity