Active Recombinant Human CR1 protein, His-tagged

• Cat.No.: CR1-506H
• Product Overview: Recombinant Human CR1(Gln42-Asp1971) fused with a C-terminal 10-His tag and a substitution of His1208Arg was expressed in NS0.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: His
• Predicted N Terminal: Gln42 predicted
• Form: Lyophilized from a 0.2 μm filtered solution in PBS.
• Bio-activity: Measured by its binding ability in a functional ELISA. When rhCD35 is coated at 5 μg/mL, complement component C3b binds with an apparent KD <10 nM.
• Molecular Mass: Predicted Molecular Mass: 213 kDa;SDS-PAGE: 200-220 kDa, reducing conditions.
• Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Storage: Avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 400 μg/mL in PBS.
• Protein length: 42-1971 a.a.

Gene Information

• Gene Name: CR1 complement component (3b/4b) receptor 1 (Knops blood group) [ Homo sapiens ]
• Official Symbol: CR1
• Synonyms: CR1; complement component (3b/4b) receptor 1 (Knops blood group); complement component (3b/4b) receptor 1, including Knops blood group system; complement receptor type 1; CD35; KN; CD35 antigen; C3b/C4b receptor; C3-binding protein; Knops blood group antigen; complement component receptor 1; C3BR; C4BR
• Gene ID: 1378
• mRNA Refseq: NM_000573
• Protein Refseq: NP_000564
• MIM: 120620
• UniProt ID: P17927
• Chromosome Location: 1q32
• Pathway: Complement and Coagulation Cascades, organism-specific biosystem; Complement and coagulation cascades, organism-specific biosystem; Complement and coagulation cascades, conserved biosystem; Complement cascade, organism-specific biosystem; Hematopoietic cell lineage, organism-specific biosystem; Hematopoietic cell lineage, conserved biosystem; Immune System, organism-specific biosystem
• Function: complement component C3b binding; complement component C3b receptor activity; complement component C4b binding; complement component C4b receptor activity; receptor activity