Active Recombinant Human COL2A1 protein, His-tagged

• Cat.No.: COL2A1-26H
• Product Overview: Recombinant Human COL2A1(Gln26-Arg266 & Gly957-Leu1487) fused with His tag at N-terminal was expressed in CHO.

Specification

• Species: Human
• Source: CHO
• Tag: His
• Protein Length: 26-266;957-1487 a.a.
• Description: Type II collagen is an essential component of cartilage. It is essential for skeletal development and for the ability of cartilage to resist compressive forces. Type II collagen is a homotrimer of a1(II) chains. Defects in Type II collagen are the cause of numerous diseases, including chondrodysplasias and arthritis. This recombinant mini pro-a1(II) collagen consists of the N‑terminal propeptide, 108 Gly-X-Y repeats, and the C‑terminal propeptide. Proteolytic processing of the C‑terminal propeptide by the procollagen C-proteinase (BMP-1) releases chondrocalcin, a calcium binding protein.
• Predicted N Terminal: His & Asp1242
• Form: Supplied as a 0.2 μm filtered solution in Tris and NaCl.
• Bio-activity: Measured by the cleavage of its C-terminal propeptide by Recombinant Human BMP‑1/PCP. >50% of full-length Pro-Collagen II is cleaved by Recombinant Human BMP‑1/PCP.
• Molecular Mass: Predicted Molecular Mass: 78 kDa;SDS-PAGE: 92-100 kDa, 65-70 kDa, 36 kDa, reducing conditions.
• Endotoxin: <1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
• Storage: Avoid repeated freeze-thaw cycles.6 months from date of receipt, -70 centigrade as supplied.3 months, -70 centigrade under sterile conditions after opening.

Gene Information

• Gene Name: COL2A1 collagen, type II, alpha 1 [ Homo sapiens ]
• Official Symbol: COL2A1
• Synonyms: COL2A1; collagen, type II, alpha 1; collagen, type II, alpha 1 (primary osteoarthritis, spondyloepiphyseal dysplasia, congenital) , SEDC; collagen alpha-1(II) chain; chondrocalcin; cartilage collagen; alpha-1 type II collagen; collagen II, alpha-1 polypeptide; AOM; ANFH; SEDC; COL11A3; MGC131516
• Gene ID: 1280
• mRNA Refseq: NM_001844
• Protein Refseq: NP_001835
• UniProt ID: P02458
• Chromosome Location: 12q12-q13.2
• Pathway: Amoebiasis, organism-specific biosystem; Amoebiasis, conserved biosystem; Axon guidance, organism-specific biosystem; Developmental Biology, organism-specific biosystem; ECM-receptor interaction, organism-specific biosystem; ECM-receptor interaction, conserved biosystem; Endochondral Ossification, organism-specific biosystem
• Function: extracellular matrix structural constituent conferring tensile strength; identical protein binding; platelet-derived growth factor binding