Recombinant Rat Col2a1 protein, His-tagged

• Cat.No.: Col2a1-7925R
• Product Overview: Recombinant Rat Col2a1 aa. (Lys1226~Tyr1331 (Accession # P05539)) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Species: Rat
• Source: E.coli
• Tag: His
• Protein Length: Lys1226~Tyr1331
• Description: Structural component of the cartilage extracellular matrix.
• Form: Freeze-dried powder
• Molecular Mass: 15kDa as determined by SDS-PAGE reducing conditions.
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 7.1.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage Buffer: Supplied as lyophilized form in PBS, pH 7.4, containing 5% sucrose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: Col2a1 collagen type II alpha 1 chain [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Col2a1
• Synonyms: Col2a1; collagen type II alpha 1 chain; CG2A1A; COLLII; collagen alpha-1(II) chain; Procollagen II alpha 1; alpha-1 type II collagen; collagen type II (Col2A1) gene, enhancer region; collagen, type II, alpha 1; procollagen, type II, alpha 1
• Gene ID: 25412
• mRNA Refseq: NM_012929.1
• Protein Refseq: NP_037061.1
• UniProt ID: P05539