Recombinant Human COL2A1 protein(Asp1242-Leu1487), His-tagged

• Cat.No.: COL2A1-3929H
• Product Overview: Recombinant Human COL2A1 isoform 1 (P02458-2)(Asp 1242-Leu 1487), known as chondrocalcin, was expressed in HEK293, with a polyhistidine tag at the N-terminus.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Protein length: Asp1242-Leu1487
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant human COL2A1 (aa 1242-1487) , known as chondrocalcin consists of 265 amino acids and has a calculated molecular mass of 29.8 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of the protein is approximately 37 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 92 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: COL2A1 collagen, type II, alpha 1 [ Homo sapiens ]
• Official Symbol: COL2A1
• Synonyms: COL2A1; collagen, type II, alpha 1; collagen, type II, alpha 1 (primary osteoarthritis, spondyloepiphyseal dysplasia, congenital) , SEDC; collagen alpha-1(II) chain; chondrocalcin; cartilage collagen; alpha-1 type II collagen; collagen II, alpha-1 polypeptide; AOM; ANFH; SEDC; COL11A3; MGC131516
• Gene ID: 1280
• mRNA Refseq: NM_001844
• Protein Refseq: NP_001835
• UniProt ID: P02458