Active Recombinant Human CDC7 Protein, GST-Tagged

• Cat.No.: CDC7-0958H
• Product Overview: Human CDC7 (NP_003494.1, 1 a.a. - 574 a.a.) and DBF4 (NP_006707.1, 1 a.a. - 674 a.a.) full-length recombinant protein with with GST tag expressed in baculovirus infected Sf21 cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST
• Description: This gene encodes a cell division cycle protein with kinase activity that is critical for the G1/S transition. The yeast homolog is also essential for initiation of DNA replication as cell division occurs. Overexpression of this gene product may be associated with neoplastic transformation for some tumors. Multiple alternatively spliced transcript variants that encode the same protein have been detected. [provided by RefSeq, Aug 2008]
• Form: Liquid
• Bio-activity: The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000.
  Substrate: MCM2 peptide. ATP: 100 μM.
• Molecular Mass: 92 kDa
• Purity: 60 % by SDS-PAGE/CBB staining
• Applications: Functional Study
  SDS-PAGE
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)

Gene Information

• Gene Name: CDC7 cell division cycle 7 homolog (S. cerevisiae) [ Homo sapiens ]
• Official Symbol: CDC7
• Synonyms: CDC7; cell division cycle 7 homolog (S. cerevisiae); CDC7 (cell division cycle 7, S. cerevisiae, homolog) like 1, CDC7 cell division cycle 7 (S. cerevisiae), CDC7L1, cell division cycle 7 (S. cerevisiae); cell division cycle 7-related protein kinase; HsCdc7; Hsk1; huCdc7; CDC7-related kinase; cell division cycle 7-like protein 1; CDC7 (cell division cycle 7, S. cerevisiae, homolog)-like 1; CDC7L1; HsCDC7; huCDC7; MGC117361; MGC126237; MGC126238
• Gene ID: 8317
• mRNA Refseq: NM_001134419
• Protein Refseq: NP_001127891
• MIM: 603311
• UniProt ID: O00311