Recombinant Human Cell Division Cycle 7 Homolog (S. cerevisiae), GST-tagged

• Cat.No.: CDC7-997H
• Product Overview: Recombinant full-length human CDC7 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Description: CDC7 is a cell division cycle protein that is critical for the G1/S transition and initiation of DNA replication during the cell division cycle. Overexpression of CDC7 gene product may be associated with neoplastic transformation for some tumors. Inhibition of CDC7 in cancer cells impairs progression through S phase, inducing a p53-independent apoptotic cell death, whereas in normal cells, it does not affect cell viability. Inhibition of CDC7 kinase activity in cancer cells restricts DNA replication and induces apoptosis. CDC7 phosphorylates the minichromosome maintenance protein 2 (Mcm2), a component of the DNA replicative helicase needed for genome duplication.
• Applications: Western Blot
• Molecular Weight: 94 kDa
• Expression System: Sf9 insect cells using baculovirus
• Form: Recombinant protein stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
• Purity: > 70 %
• Concentration: 0.2 ug/ul
• Sequences: Full-length
• Storage: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Pathways: Activation of ATR in response to replication stress; Activation of the pre-replicative complex; Cell Cycle Checkpoints; Cell Cycle; Cell cycle; DNA Replication; G1/S Transition; G2/M Checkpoints; M/G1 Transition; Mitotic G1-G1/S phases; Mitotic M-M/G1 phases

Gene Information

• Gene Name: CDC7 cell division cycle 7 homolog (S. cerevisiae) [ Homo sapiens ]
• Official Symbol: CDC7
• Synonyms: CDC7; cell division cycle 7 homolog (S. cerevisiae); CDC7L1; Hsk1; CDC7 (cell division cycle 7, S. cerevisiae, homolog)-like 1; HsCdc7; huCdc7; CDC7-related kinase; CDC7 cell division cycle 7 (S. cerevisiae); cell division cycle 7 (S. cerevisiae); HsCDC7; MGC117361; MGC126237; MGC126238; cell division cycle 7-like protein 1; cell division cycle 7-related protein kinase; huCDC7; EC 2.7.11.1
• Gene ID: 8317
• mRNA Refseq: NM_003503
• Protein Refseq: NP_003494
• MIM: 603311
• UniProt ID: O00311
• Chromosome Location: 1p22
• Function: ATP binding; kinase activity; metal ion binding; nucleotide binding; protein serine/threonine kinase activity