Active Recombinant Human CD27 Protein, Fc-tagged, Alexa Fluor 555 conjugated

• Cat.No.: CD27-180HAF555
• Product Overview: Alexa Fluor 555 conjugated recombinant human CD27 (P26842) (Met1-Ile192) was expressed, fused with the Fc region of rabbit IgG at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 396
• Form: Lyophilized
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized human CD27-his at 10 μg/mL (100 μL/well) can bind biotinylated human CD70-Fc, The EC50 of biotinylated human CD70-Fc is 95-150 ng/mL.
• Molecular Mass: The recombinant human CD27/rFc is a disulfide-linked homodimer. The reduced monomer comprises 396 amino acids and has a predicted molecular mass of 44.4 kDa. The apparent molecular mass of the protein is approximately 63 kDa in SDS-PAGE under reducing conditions.
• N-terminal Sequence Analysis: Ala 20
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 to-70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile PBS, pH 7.4, 5%-8% trehalose and mannitol.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: CD27 CD27 molecule [ Homo sapiens ]
• Official Symbol: CD27
• Synonyms: CD27; CD27 molecule; TNFRSF7, tumor necrosis factor receptor superfamily, member 7; CD27 antigen; S152; Tp55; CD27L receptor; T cell activation antigen S152; T-cell activation antigen CD27; tumor necrosis factor receptor superfamily, member 7; T14; TNFRSF7; MGC20393
• Gene ID: 939
• mRNA Refseq: NM_001242
• Protein Refseq: NP_001233
• MIM: 186711
• UniProt ID: P26842