Recombinant Human CD27 Protein, Fc/His-tagged, Alexa Fluor 488 conjugated

• Cat.No.: CD27-638HAF488
• Product Overview: Alexa Fluor 488 conjugated recombinant human CD27 extracellular domain (Met 1-Ile 192) (NP_001233.1), fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus, was produced in Human Cell.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc&His
• Protein Length: 420
• Form: Lyophilized
• Molecular Mass: The recombinant mature human CD27/Fc is a disulfide-linked homodimeric protein. The reduced monomer comprises 420 amino acids and has a calculated molecular mass of 47.2 kDa. As a result of glycosylation, the monomer migrates as an approximately 65 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 488 via amines
  Excitation Wavelength: 488 nm
  Emission Wavelength: 515­-545 nm
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 to -70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile PBS, pH 7.4
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: CD27 CD27 molecule [ Homo sapiens ]
• Official Symbol: CD27
• Gene ID: 939
• mRNA Refseq: NM_001242
• Protein Refseq: NP_001233
• MIM: 186711
• UniProt ID: P26842