Active Recombinant Human CCN4 Protein

• Cat.No.: CCN4-197C
• Product Overview: Recombinant Human CCN4 Protein without tag was expressed in CHO.

Specification

• Description: WISP-1, also known as Wnt-1-inducible-signaling pathway protein 1, CCN4 and Wnt-1-induced secreted protein, is a cysteine-rich heparin-binding Glycoprotein belonging to the CCN protein family. It is expressed in many internal organs, such as the lung, kidney and spleen. WISP-1 binds to BMP-2 and enhances mesenchymal cell proliferation and osteoblastic differentiation., WISP-1 has also been reported to attenuate p53-mediated apoptosis and inhibit TNF-induced cell death, suggesting it may play a role intumorigenesis.
• Source: CHO
• Species: Human
• Form: Sterile Filtered White lyophilized (freeze-dried) powder.
• Bio-activity: ED50< 0.5 μg/mL, measured in a bioassay using ATDC5 cells in the presence of 50 ng/mL human BMP-2.
• Molecular Mass: 44-46 kDa, observed by reducing SDS-PAGE.
• AA Sequence: TALSPAPTTMDFTPAPLEDTSSRPQFCKWPCECPPSPPRCPLGVSLITDGCECCKMCAQQLGDNCTEAAICDPHRGLYCDYSGDRPRYAIGVCAQVVGVGCVLDGVRYNNGQSFQPNCKYNCTCIDGAVGCTPLCLRVRPPRLWCPHPRRVSIPGHCCEQWVCEDDAKRPRKTAPRDTGAFDAVGEVEAWHRNCIAYTSPWSPCSTSCGLGVSTRISNVNAQCWPEQESRLCNLRPCDVDIHTLIKAGKKCLAVYQPEASMNFTLAGCISTRSYQPKYCGVCMDNRCCIPYKSKTIDVSFQCPDGLGFSRQVLWINACFCNLSCRNPNDIFADLESYPDFSEIAN
• Endotoxin: < 0.2 EU/μg, determined by LAL method.
• Purity: > 95% as analyzed by SDS-PAGE.
• Storage: Lyophilized recombinant human WISP-1 remains stable up to 6 months at -80 centigrade from date of receipt. Upon reconstitution, human WISP-1 should be stable up to 1 week at 4 centigrade or up to 2 months at -20 centigrade.
• Storage Buffer: Lyophilized after extensive dialysis against PBS.
• Reconstitution: Reconstituted in ddH2O or PBS at 100 μg/mL.

Gene Information

• Gene Name: CCN4 cellular communication network factor 4 [ Homo sapiens (human) ]
• Official Symbol: CCN4
• Synonyms: CCN4; cellular communication network factor 4; WISP1; WISP1c; WISP1i; WISP1tc; WISP1-OT1; WISP1-UT1
• Gene ID: 8840
• mRNA Refseq: NM_080838
• Protein Refseq: NP_543028
• MIM: 603398
• UniProt ID: O95388