Recombinant Human cellular communication network factor 4 Protein, His tagged

• Cat.No.: CCN4-001H
• Product Overview: Recombinant human cellular communication network factor 4 Protein (23-367 aa) wiht C-His tag was expressed in E. coli.

Specification

• Description: This gene encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like domain. This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. It is expressed at a high level in fibroblast cells, and overexpressed in colon tumors. The encoded protein binds to decorin and biglycan, two members of a family of small leucine-rich proteoglycans present in the extracellular matrix of connective tissue, and possibly prevents the inhibitory activity of decorin and biglycan in tumor cell proliferation. It also attenuates p53-mediated apoptosis in response to DNA damage through activation of the Akt kinase. It is 83% identical to the mouse protein at the amino acid level. Multiple alternatively spliced transcript variants have been identified.
• Source: E. coli
• Species: Human
• Tag: C-His
• Protein length: 23-367aa
• Molecular Mass: 39 kDa
• AA Sequence: MTALSPAPTTMDFTPAPLEDTSSRPQFCKWPCECPPSPPRCPLGVSLITDGCECCKMCAQQLGDNCTEAAICDPHRGLYCDYSGDRPRYAIGVCAQVVGVGCVLDGVRYNNGQSFQPNCKYNCTCIDGAVGCTPLCLRVRPPRLWCPHPRRVSIPGHCCEQWVCEDDAKRPRKTAPRDTGAFDAVGEVEAWHRNCIAYTSPWSPCSTSCGLGVSTRISNVNAQCWPEQESRLCNLRPCDVDIHTLIKAGKKCLAVYQPEASMNFTLAGCISTRSYQPKYCGVCMDNRCCIPYKSKTIDVSFQCPDGLGFSRQVLWINACFCNLSCRNPNDIFADLESYPDFSEIANHHHHHHHH
• Endotoxin: < 1 EU/μg of protein (determined by LAL method)
• Purity: > 90% by SDS-PAGE
• Storage: Store it under sterile conditions at -20 to -80 centigrade. Easy to precipitate, Please Do Not Keep at 4 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Sterile PBS, pH 7.4, 10% Glycerol
• Concentration: 0.58 mg/mL by BCA

Gene Information

• Gene Name: CCN4 cellular communication network factor 4 [ Homo sapiens (human) ]
• Official Symbol: CCN4
• Synonyms: CCN4; cellular communication network factor 4; WISP1; WISP1c; WISP1i; WISP1tc; WISP1-OT1; WISP1-UT1
• Gene ID: 8840
• mRNA Refseq: NM_080838
• Protein Refseq: NP_543028
• MIM: 603398
• UniProt ID: O95388