Active Recombinant Human BTD protein, His-tagged

• Cat.No.: BTD-0258H
• Product Overview: Recombinant Human BTD protein(P43251)(Met1-Asp543), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to hydrolyze biocytin to lysine and biotin. The specific activity is >500pmol/min/μg.
• Molecular Mass: The recombinant human BTD comprises 513 amino acids and has a predicted molecular mass of 58.2 kDa. The apparent molecular mass of the protein is approximately 66-76 kDa in SDS-PAGE under reducing conditions.
• Protein length: Met1-Asp543
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: BTD biotinidase [ Homo sapiens ]
• Official Symbol: BTD
• Synonyms: BTD; biotinidase; biotinase
• Gene ID: 686
• mRNA Refseq: NM_000060
• Protein Refseq: NP_000051
• MIM: 609019
• UniProt ID: P43251