Recombinant Human BTD protein, His-tagged

• Cat.No.: BTD-1681H
• Product Overview: Recombinant Human BTD aa. (Ala42~Ala271) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: The protein encoded by this gene functions to recycle protein-bound biotin by cleaving biocytin (biotin-epsilon-lysine), a normal product of carboxylase degradation, resulting in regeneration of free biotin. The encoded protein has also been shown to have biotinyl transferase activity. Mutations in this gene are associated with biotinidase deficiency. Multiple transcript variants encoding different isoforms have been described.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: 30kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Ala42~Ala271
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >97%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Isoelectric Point: 5.4

Gene Information

• Gene Name: BTD biotinidase [ Homo sapiens (human) ]
• Official Symbol: BTD
• Synonyms: BTD; biotinidase; biotinase
• Gene ID: 686
• mRNA Refseq: NM_000060.4
• Protein Refseq: NP_000051.1
• UniProt ID: P43251