Active Recombinant Human ATXN3L, His-tagged

Description :	There are two main classes of DUB; cysteine proteases and metalloproteases. Ataxin-3L is a cysteine protease and a member of the Machado-Joseph Domain (MJD) enzyme family. Cloning of the human gene was first described by Gerhard et al. (2004). Machado–Joseph disease (MJD), the most common form of spinocerebellar ataxia worldwide, is a progressive and ultimately fatal neurodegenerative disorder caused by polyQ expansion in ataxin-3, a conserved and ubiquitous protein known to bind polyubiquitin chains and to function as a deubiquitylating enzyme.
Source :	E. coli
Species :	Human
Tag :	His
Form :	50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol
Bio-activity :	Deubiquitylase Enzyme Assay: The activity of His-Ataxin-3L was validated by determining the increase in fluorescence measured as a result of the enzyme catalysed cleavage of the fluorogenic substrate Ubiquitin-Rhodamine110-Glycine generating Ubiquitin and Rhodamine110-Glycine. Incubation of the substrate in the presence or absence of His-Ataxin-3L was compared confirming the deubiquitylating activity of His-Ataxin-3L.
Molecular Mass :	~43 kDa
Purity :	>92% by SDS-PAGE
Storage :	12 months at -70°C. Avoid multiple freeze/thaw cycles.
Concentration :	0.5 mg/ml
Protein length :	1-355 a.a.

Gene Name	ATXN3L ataxin 3-like [ Homo sapiens ]
Official Symbol	ATXN3L
Synonyms	ataxin 3-like; ATX3L; MJDL; EC 3.4.19.12; Machado-Joseph disease protein 1-like; EC 3.4.22; putative ataxin-3-like protein
Gene ID	92552
mRNA Refseq	NM_001135995.1
Protein Refseq	NP_001129467.1
UniProt ID	B4DYC7
Chromosome Location	Xp22.2
Pathway	Protein processing in endoplasmic reticulum, organism-specific biosystem; Protein processing in endoplasmic reticulum, conserved biosystem
Function	omega peptidase activity; ubiquitin-specific protease activity