Active Recombinant Human ATG7 Protein, His-tagged

• Cat.No.: ATG7-1076H
• Product Overview: Recombinant Human ATG7 (Accession # O95352), fused with an N-terminal 6-His tag, was produced in Spodoptera frugiperda, Sf 21 (baculovirus)-derived.

Specification

• Source: Baculovirus Sf21 Insect Cells
• Species: Human
• Tag: His
• Form: X mg/ml (X µM) in 50 mM HEPES pH 7.0, 150 mM NaCl, 2 mM TCEP, 10% glycerol
• Bio-activity: Recombinant Human His6-ATG7 (Apg7) is a member of the Apg-activating (E1) enzyme family that is required for the first step of the enzymatic cascade that subsequently utilizes a Apg-conjugating (E2) enzyme and a Apg ligase (E3) to conjugate Apg to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-ATG7 (Apg7) concentration of 50-200 nM.
• Molecular Mass: Recombinant Human ATG7, His-tagged has a calculated MW of 80 kDa.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: ATG7 ATG7 autophagy related 7 homolog (S. cerevisiae) [ Homo sapiens ]
• Official Symbol: ATG7
• Synonyms: ATG7; ATG7 autophagy related 7 homolog (S. cerevisiae); APG7 autophagy 7 like (S. cerevisiae) , APG7L; ubiquitin-like modifier-activating enzyme ATG7; DKFZp434N0735; GSA7; hAGP7; autophagy-related protein 7; ATG12-activating enzyme E1 ATG7; ubiquitin activating enzyme E1-like protein; ubiquitin-activating enzyme E1-like protein; APG7L; APG7-LIKE
• Gene ID: 10533
• mRNA Refseq: NM_001136031
• Protein Refseq: NP_001129503
• MIM: 608760
• UniProt ID: O95352
• Chromosome Location: 3p25.3-p25.2