Active Recombinant Human ALPL Protein, His-tagged

• Cat.No.: ALPL-327H
• Product Overview: Recombinant Human ALPL Protein (Leu18-Ser502) is produced by NS0-derived expression system. This protein is fused with a His tag at the C-terminal.

Specification

• Description: Four distinct genes encode alkaline phosphatases (APs) in humans (1). The ALPL gene encodes the liver/bone/kidney isozyme, also known as the tissue‑nonspecific AP (TNAP). In comparison, ALPI, ALPP and ALPPL2 encode intestinal, placental and placental-like or germ cell APs, respectively. The serum levels of human APs are useful tumor markers (2). There are many mutations in the ALPL gene, leading to different forms of hypophosphatasia, characterized by poorly mineralized cartilage and bones (3). The native ALPL is a glycosylated homodimer attached to the membrane through a GPI-anchor. The C-terminal pro peptide (residues 503 to 524) is not present in the mature form.
• Source: Mammalian cells
• Species: Human
• Tag: His
• Predicted N Terminal: Leu18
• Form: Supplied as a 0.2 μm filtered solution in HEPES, NaCl, MgCl2 and ZnCl2.
• Bio-activity: Measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl phosphate (4-MUP). The specific activity is >30,000 pmol/min/µg.
• Molecular Mass: 54 kDa
• Endotoxin: <1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Avoid repeated freeze-thaw cycles.
  6 months from date of receipt, -20 to -70 centigrade as supplied.
  3 months, -20 to -70 centigrade under sterile conditions after opening.
• Protein length: 18-502 a.a.

Gene Information

• Gene Name: ALPL alkaline phosphatase, liver/bone/kidney [ Homo sapiens ]
• Official Symbol: ALPL
• Synonyms: Akp2; ALPL; APTNAP; AP-TNAP; EC 3.1.3.1; FLJ40094; FLJ93059; MGC161443; tissue-nonspecific ALP; TNAP; TNSALP; TNSALPMGC167935
• Gene ID: 249
• mRNA Refseq: NM_000478
• Protein Refseq: NP_000469
• MIM: 171760
• UniProt ID: P05186