Active Recombinant Human ADAM23 Protein, His-tagged

• Cat.No.: ADAM23-1025H
• Product Overview: Recombinant Human ADAM23 (Accession # O75077) Ser60-His585, fused with a C-terminal 10-His tag, was produced in Chinese Hamster Ovary cell line, CHO-derived.

Specification

• Source: CHO Cells
• Species: Human
• Tag: His
• Predicted N Terminal: Ser60 (pro) & Ala287 (mature)
• Form: Lyophilized from a 0.2 µm filtered solution in Tris and NaCl.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of BCE C/D-1b bovine corneal endothelial cells.The ED50 for this effect is typically 1.0-4.0 µg/mL.
• Molecular Mass: Recombinant Human ADAM23, His-tagged has a calculated MW of 60.8 kDa (pro) & 34.9 kDa (mature). In SDS-PAGE migrates as 40-60 kDa, reducing conditions.
• Protein length: Ser60-His585
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 100 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: ADAM23 ADAM metallopeptidase domain 23 [ Homo sapiens ]
• Official Symbol: ADAM23
• Synonyms: ADAM23; ADAM metallopeptidase domain 23; a disintegrin and metalloproteinase domain 23; disintegrin and metalloproteinase domain-containing protein 23; MDC3; MDC-3; metalloproteinase-like, disintegrin-like, and cysteine-rich protein 3
• Gene ID: 8745
• mRNA Refseq: NM_003812
• Protein Refseq: NP_003803
• MIM: 603710
• UniProt ID: O75077
• Chromosome Location: 2q33