Active Recombinant Full Length Human EIF2AK4 Protein, GST-tagged

Cat.No. : EIF2AK4-3747HF
Product Overview : Full-length human GCN2 [1-1649(end) amino acids and E556G of accession number NP_001013725] was expressed as N-terminal GST-fusion protein (215 kDa) using baculovirus expression system. GST- GCN2 was purified by using glutathione sepharose chromatography.
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Source : Insect (sf21)
Species : Human
Tag : GST
Form : 50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
Bio-activity : The activity was determined by ELISA. The enzyme was incubated with GST-fused substrate protein and tRNA, and after stopping kinase reaction by EDTA, the reaction solution was transferred into glutathione-coated plate. Phosphorylation was detected by anti- phospho antibody and HRP-labeled anti-rabbit IgG.
Protein length : 1-1649(end)
Purity : 82%
Applications : SDS-PAGE
Storage : Store at -80C. Avoid repeating freeze-thaws.
Gene Name eukaryotic translation initiation factor 2 alpha kinase 4
Official Symbol EIF2AK4
Synonyms EIF2AK4;GCN2;PVOD2;KIAA1338
Protein Refseq NP_001013725
UniProt ID Q9P2K8

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