Active Recombinant Feline VEGFA, Met-tagged

• Cat.No.: VEGFA-637F
• Product Overview: Recombinant Feline VEGFA (Accession # NP_001009854) Ala27-Arg189, fused with an N-terminal Met, was produced in E. coli-derived.

Specification

• Species: Feline
• Source: E.coli
• Protein Length: 27-189 a.a.
• Predicted N Terminal: Met & Pro28
• Form: Lyophilized from a 0.2 µm filtered solution in HCl.
• Bio-activity: Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. Conn, G. et al. (1990) Proc. Natl. Acad. Sci. USA 87:1323.The ED50 for this effect is typically 1.2 - 6 ng/mL.
• Molecular Mass: Recombinant Feline VEGFA, Met-tagged has a calculated MW of 19.2 kDa (monomer). In SDS-PAGE migrates as 20 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: VEGFA vascular endothelial growth factor A [ Felis catus (domestic cat) ]
• Official Symbol: VEGFA
• Synonyms: VEGFA; vascular endothelial growth factor A; VEGF
• Gene ID: 493845
• mRNA Refseq: NM_001009854
• Protein Refseq: NP_001009854
• Chromosome Location: B2