Active Recombinant Cynomolgus TNFSF4, Fc-tagged

• Cat.No.: TNFSF4-85C
• Product Overview: A DNA sequence encoding the cynomolgus TNFSF4 (Gln51-Leu183) was expressed with the Fc region of mouse IgG1 at the N-terminus.

Specification

• Description: OX-40L, also known as TNFSF4 and CD252, is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. OX-40L is an important costimulatory molecule that plays a crucial role in the regulation of T-cell-mediated immunity. The interaction of TNFSF4-TNFSF4 is involved in the pathogenesis of multiple autoimmune and inflammatory diseases such as systemic lupus erythematosus (SLE), carotid artery disease and cancer. OX-40L is a ligand for receptor TNFRSF4/OX4. It is found to play a role in T cell antigen-presenting cell (APC) interactions. In surface Ig- and CD40-stimulated B cells, this cytokine along with CD70 has been shown to provide CD28-independent costimulatory signals to T cells. This protein and its receptor are reported to directly mediate adhesion of activated T cells to vascular endothelial cells.
• Source: Human Cells
• Species: Cynomolgus
• Tag: Fc
• Predicted N Terminal: Asp
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Immobilized Cynomolgus mFc-TNFSF4 at 10 μg/ml (100 μl/well) can bind human TNFRSF4-Fch, The EC50 of human TNFRSF4-Fch is 0.23-0.55 μg/ml.
• Molecular Mass: The recombinant cynomolgus TNFSF4 is a disulfide-linked homodimer. The reduced monomer comprises 369 amino acids and has a calculated molecular mass of 42 KDa.The apparent molecular mass of it is approximately 48 KDa respectively in SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 85 % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products.
• Protein length: 51-183 a.a.

Gene Information

• Gene Name: TNFSF4 tumor necrosis factor (ligand) superfamily, member 4 [ Macaca fascicularis (crab-eating macaque) ]
• Official Symbol: TNFSF4
• Synonyms: TNFSF4; tumor necrosis factor (ligand) superfamily, member 4; hypothetical protein
• Gene ID: 102141877
• mRNA Refseq: XM_005540022
• Protein Refseq: XP_005540079
• Chromosome Location: 1
• Pathway: Cytokine-cytokine receptor interaction