Active Recombinant Cynomolgus/Rhesus macaque TNFSF4 protein, His-tagged

• Cat.No.: TNFSF4-0586C
• Product Overview: Recombinant Cynomolgus/Rhesus macaque TNFSF4 protein(Gln51-Leu183), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Cynomolgus/Rhesus macaque
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Immobilized Cynomolgus/Rhesus macaque OX40 Ligand, His Tag at 2 μg/mL (100 μL/well) can bind Cynomolgus / Rhesus macaque OX40, Fc Tag with a linear range of 0.1-3 ng/mL. Immobilized Cynomolgus/Rhesus macaque OX40 Ligand, His Tag at 2 μg/mL (100 μL/well) can bind Human OX40, Fc Tag with a linear range of 0.1-1 ng/mL. Immobilized Cynomolgus / Rhesus macaque OX40, Fc Tag at 5 μg/mL (100 μL/well) can bind Cynomolgus/Rhesus macaque OX40 Ligand, His Tag with a linear range of 20-78 ng/mL.
• Molecular Mass: Molecular CharacterizationThis protein carries a polyhistidine tag at the N-terminusThe protein has a calculated MW of 49.3 kDa. The protein migrates as 65-80 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Gln51-Leu183
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE. >95% as determined by SEC-MALS.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Protein length: 51-183 a.a.