Active Recombinant Cynomolgus FCGRT & B2M protein (Met 1-Met 119), His-tagged

• Cat.No.: FCGRT & B2M-1533C
• Product Overview: Recombinant cynomolgus FCGRT (Q8SPV9) extracellular domain (Met 1-Ser 297) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; Recombinant cynomolgus B2M (Q8SPW0) (Met 1-Met 119) constructed the plasmid 2. The two plasmids were co-expressed and the FCGRT/B2M heterodimer was purified.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: His
• Protein Length: 1-297 a.a.
• Predicted N Terminal: Ala 24 & Ile 21
• Form: Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.
• Bio-activity: Measured by its ability to bind human IgG1 in functional ELISA.
• Molecular Mass: The recombinant heterodimer of cynomolgus FCGRT&B2M comprises 384 (285+99) amino acids and has a calculated molecular mass of 43.5 (31.9+ 11.6) KDa. The apparent molecular mass of cynomolgus FCGRT&B2M heterodimer is approximately 35 & 12 KDa respectively in SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 98 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 mg/ml. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Gene Information

• Official Symbol: FCGRT
• Synonyms: Fc fragment of IgG, receptor, transporter, alpha