Active Recombinant Canine NRG1

• Cat.No.: NRG1-178C
• Product Overview: A DNA sequence encoding the canine NRG1 isoform alpha (XP_858147.1) extracellular domain (Ser19-Lys240) was expressed with five amino acids (DDDDK) at the C- terminus.

Specification

• Species: Canine
• Source: Human Cells
• Tag: Non
• Protein Length: Ser19-Lys240
• Form: Lyophilized from sterile PBS, pH7.4.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized canine NRG1 at 10 μg/ml (100 μl/well) can bind human ErbB4-Fc, The EC50 of human ErbB4-Fc is 0.19-0.43 μg/ml.
• Molecular Mass: The recombinant canine NRG1 comprises 228 amino acids and has a predicted molecular mass of 24.7 kDa. The apparent molecular mass of the protein is approximately 47 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 eu per μg of the protein as determined by the LAL method.
• Purity: >95 % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: Hardcopy of COA with reconstitution instruction is sent along with the products.

Gene Information

• Gene Name: NRG1 neuregulin 1 [ Canis lupus familiaris ]
• Official Symbol: NRG1
• Synonyms: NRG1; neuregulin 1; pro-neuregulin-1, membrane-bound isoform
• Gene ID: 482861
• Pathway: ErbB signaling pathway, organism-specific biosystem; ErbB signaling pathway, conserved biosystem